Mouse Cytochrome b5 (Cyb5a) ELISA Kit

Designed for the quantitative measurement of cytochrome b5 in mouse research samples, this Assay Genie sandwich ELISA kit is reactive with mouse and is suitable for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is reported for natural and recombinant mouse cytochrome b5 (Cyb5a).

Cytochrome b5 is a membrane-bound hemoprotein that functions as an electron carrier for several membrane-bound oxygenases and participates in the C-5 desaturation step of sterol biosynthesis. Dysregulation of cytochrome b5 has been associated with metabolic disorders and neurological conditions, supporting its use as a biomarker in related research.

Analytical characteristics include a detection range of 31.2-2000 pg/mL, a sensitivity of 15.64 pg/mL, an intra-assay CV of 5.4% and an inter-assay CV of 7.7%. Reported linearity values range between 87% and 119% across serum, EDTA plasma and heparin plasma dilutions, with average recovery of 81% for serum and 83% for plasma. For research use only.