Mouse Cystatin 3 (CST3) ELISA Kit

For quantitative measurement of Cystatin C in murine research samples, the Assay Genie Mouse Cystatin 3 (CST3) ELISA Kit delivers an absorbance-based, two-antibody immunoassay formatted on a 96-assay microplate. Cystatin 3 (also called cystatin C) is a ~13 kDa cysteine protease inhibitor that suppresses cathepsin B, H, L, and S activity and functions as an endogenous readout of glomerular filtration in mouse models.

Investigators apply the kit to study renal function, cerebral amyloid angiopathy, neurodegeneration, and inflammatory pathways where CST3 expression is altered. The assay accommodates serum or plasma, as well as tissue homogenates, cell lysates, and cell culture supernatants, supporting comparisons between wild-type and transgenic mice, time-course studies, and treatment-effect analyses across tissues.

Each kit provides a pre-coated plate, reference standards, a matched detection antibody, and substrate reagents sufficient for 96 determinations. Researchers typically run samples in duplicate against a standard curve, aliquot reagents upon arrival to limit freeze-thaw cycles, and store the kit refrigerated between uses. For research use only; not for clinical diagnostic procedures.