Mouse CYP7A1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is a specialized assay designed for the precise measurement of CYP7A1 levels in mouse samples. CYP7A1, also known as cholesterol 7 alpha-hydroxylase, is a key enzyme involved in bile acid synthesis and cholesterol metabolism. Dysregulation of CYP7A1 has been linked to metabolic disorders including hypercholesterolemia and gallstone formation, and the kit supports research into cardiovascular health, liver function, and metabolic disorders in mouse models.

The assay uses a Sandwich ELISA, Double Antibody configuration with colorimetric detection by adding stop solution and reading absorbance at 450 nm after TMB colour development. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 0.188 ng/mL and the assay range is 0.313-20 ng/mL. Supplied components include a dismountable ELISA microplate, lyophilized standard, biotin-labelled detection antibody, HRP-Streptavidin conjugate (SABC), TMB substrate, sample, antibody and SABC dilution buffers, stop solution, 25X wash buffer, plate sealer, and a technical manual.

Reported linearity for serum (n=5) is 87-105% at 1:2, 83-100% at 1:4, and 81-95% at 1:8; for EDTA plasma (n=5) 86-104%, 88-98%, and 81-93%; for heparin plasma (n=5) 86-104%, 82-95%, and 81-99%. Recovery ranges are 89-105% (average 95%) for serum, 91-105% (average 98%) for EDTA plasma, and 90-98% (average 94%) for heparin plasma. The kit is stored at 2-8°C for 12 months. Listed target synonyms include CYPVII and cholesterol 7-alpha-monooxygenase.