Mouse CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) ELISA Kit (MOES01742)

This Assay Genie kit enables quantitative measurement of CYP2E1, a xenobiotic-metabolizing heme-thiolate enzyme predominantly expressed in liver microsomes. CYP2E1 oxidizes ethanol, acetone, acetaminophen, and various industrial solvents, and its induction has been linked with oxidative stress, alcoholic liver disease, and chemically mediated hepatotoxicity in murine models. The protocol accommodates 50 uL sample volumes and is appropriate for studies of drug-drug interactions, fasting-induced steatosis, and cytochrome-mediated bioactivation. Researchers tracking hepatic injury biomarkers in knockout or diet-induced obese mouse cohorts routinely apply this quantitative readout across tissue homogenates and biofluids.