Mouse Cyclophilin B (CYPB) ELISA Kit

This research-use immunoassay from Assay Genie enables quantitative measurement of Cyclophilin B (CYPB), a 22 kDa endoplasmic reticulum chaperone that catalyzes peptidyl-prolyl cis-trans isomerization and supports folding of proline-containing proteins, including collagen precursors. CYPB additionally binds cyclosporin A and participates in calcium signaling, making it a relevant biomarker in studies of ER stress, fibrosis, connective tissue disease, and immune modulation.

The microplate-based protocol accommodates 96 determinations per box and includes pre-coated wells, detection antibodies, substrate reagents, and reference standards for use in vitro. Researchers investigating transplant biology, chaperone function, or matrix remodeling can measure CYPB concentrations in serum samples, plasma samples, cell lysates, and tissue homogenates. Standard curves are generated in parallel with samples to enable interpolation of unknown concentrations.

For storage, keep unopened components refrigerated and protect from light to preserve antibody activity. Bring all reagents to room temperature before initiating the assay, and avoid repeated freeze-thaw cycles with reconstituted standards. Reconstituted working solutions should be used within the period specified in the product insert.