Mouse Cyclophilin A (CYPA) ELISA Kit

This microplate-format immunoassay from Assay Genie provides quantitative measurement of mouse Cyclophilin A (CypA), an 18 kDa peptidyl-prolyl cis-trans isomerase implicated in protein folding, inflammatory signaling, chemokine receptor trafficking, and cardiovascular remodeling. Researchers apply it in vitro across immunology, infectious disease, vascular biology, and cell-signaling studies where CypA is investigated as a candidate biomarker or a normalization reference.

Reagents are supplied in vials sized for a single-use plate workflow, with capture antibody pre-immobilized on the wells and chromogenic detection reagents packaged separately. Working standards and samples are dispensed in microliter volumes, and bound analyte is quantified by measuring substrate absorbance on a standard plate reader. The kit supports ELISA-based experiments on specimens recovered from mouse models, including tissue homogenates, cell lysates, and cell culture supernatant preparations recovered under varied experimental conditions.

Component vials should be kept refrigerated upon arrival and stored protected from light, with working standards aliquoted into smaller portions to limit freeze-thaw cycles over the kit's shelf life. Once reconstituted, individual reagents perform within the assay's reported dynamic range when standard curves are freshly prepared per run. Intended for research use only.