Mouse Cyclin Dependent Kinase 8 (CDK8) ELISA Kit

Quantify mouse Cyclin Dependent Kinase 8 (CDK8) protein levels with this in vitro ELISA from Assay Genie, supplied as a 96-assay microplate kit calibrated for tissue homogenate samples. CDK8 acts as a serine/threonine kinase within the Mediator complex alongside cyclin C, MED12, and MED13, phosphorylating transcription factors and the C-terminal domain of RNA polymerase II to modulate gene expression. The assay supports research into Wnt/β-catenin and p53 signaling pathways, and into disease contexts where CDK8 dysregulation has been implicated, including colorectal cancer, breast cancer, and acute myeloid leukemia. Standard workflow involves preparing recombinant protein standard curves, loading samples and standards onto the pre-coated plate, sequential incubation with detection reagents, washing, and color development with TMB substrate. Researchers can compare CDK8 concentrations across experimental conditions, tissues, genotypes, or pharmacological treatments. Each kit ships with a pre-coated microplate, detection antibody concentrate, recombinant standards, standard diluent, assay buffers, wash solution, TMB substrate, and stop reagent, accompanied by a comprehensive protocol leaflet.