Mouse Cyb5r3 ELISA Kit

From Assay Genie, this Sandwich ELISA kit based on the double antibody method is designed for the detection and measurement of CYB5R3 in mouse samples. CYB5R3 is a key enzyme involved in electron transport and redox reactions, playing a critical role in various metabolic processes and cellular functions. Dysregulation of CYB5R3 has been linked to disorders such as neurological diseases, metabolic disorders, and cancer, highlighting its importance as a potential biomarker for disease research and therapeutic development.

The kit is reactive with mouse and validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, with TMB substrate development and absorbance read at 450 nm. The kit is stored at 2-8°C for 12 months. The associated UniProt identifier is Q9DCN2 and the KEGG ID is K00326.

Reported linearity values (n=5) at 1:2, 1:4, and 1:8 dilutions are 89-103%, 88-101%, and 86-104% for serum; 88-101%, 82-99%, and 84-97% for EDTA plasma; and 90-100%, 85-100%, and 84-93% for heparin plasma. Recovery ranges are 86-100% (average 94%) for serum, 91-104% (average 99%) for EDTA plasma, and 91-102% (average 97%) for heparin plasma. The procedure involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and immediate absorbance measurement after stop solution addition.