Mouse CYB561 ELISA Kit

Assay Genie supplies a sandwich ELISA, double antibody kit designed for quantitative measurement of mouse CYB561. The kit is reactive with mouse and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a colorimetric detection read at 450 nm. The manufacturer reports a sensitivity of 0.938 ng/mL and a working range of 1.563–100 ng/mL, with the associated UniProt entry Q60720. Reagents are stored at 2–8°C for 12 months.

According to the manufacturer, linearity across 1:2, 1:4 and 1:8 dilutions falls within 82–105% for serum, EDTA plasma and heparin plasma (n=5 each), and recovery ranges are 92–104% (average 98%) for serum, 87–104% (average 98%) for EDTA plasma, and 85–101% (average 93%) for heparin plasma. The protocol calls for a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-streptavidin (SABC) binding step, and TMB development in the dark for 10–20 minutes, followed by stop solution and immediate absorbance reading at 450 nm.

Assay Genie literature describes CYB561 as a protein involved in cellular redox regulation and electron transport, with research interest in neurodegenerative disorders, cardiovascular diseases and metabolic disorders.