Mouse CXCL6/GCP-2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of mouse CXCL6, also known as GCP-2 (Granulocyte Chemotactic Protein 2). The target is a chemokine that plays a central role in the inflammatory response by recruiting leukocytes to sites of infection or injury, making it a useful biomarker for inflammation-related research.

Validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, the kit uses a colorimetric detection method. It delivers a sensitivity of 18.75 pg/mL across a working range of 31.25-2000 pg/mL, and all components are stored at 2-8°C for 12 months. Reported linearity spans 80-105% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, while recovery averages 95% for serum, 98% for EDTA plasma, and 93% for heparin plasma.

The double-antibody sandwich workflow pairs a biotin-labeled detection antibody with HRP-streptavidin binding, followed by TMB substrate development and absorbance reading at 450 nm. Additional target synonyms include GCP2, SCYB6, and CKA-3.