Mouse CTSL (Cathepsin L) ELISA Kit (MOES00821)

From Assay Genie, this Sandwich-ELISA kit is intended for the quantitative measurement of mouse Cathepsin L (CTSL) in serum, plasma, and other biological fluids. The assay uses a colorimetric sandwich format in which samples bind to a pre-coated capture antibody, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. Optical density is read at 450 nm ± 2 nm and the target concentration is determined from a standard curve. The recommended sample volume is 100 μL and the total assay time is 3 h 30 min.

Performance characteristics reported by the manufacturer include a sensitivity of 18.75 pg/mL, a detection range of 31.25-2000 pg/mL, and a recovery of 80%-120%. The kit specifically recognises mouse CTSL, with no significant cross-reactivity or interference between mouse CTSL and analogues observed. Storage is at 2-8°C. Indicated research areas are cancer, neuroscience, and signal transduction. Synonyms listed for the target include CTSL1, CATL, and MEP, and the associated UniProt ID is P06797.