Mouse CTSB (Cathepsin B) ELISA Kit (MOES01703)

Assay Genie offers a colorimetric sandwich-format ELISA kit for the quantitative detection of cathepsin B (CTSB) in mouse samples, with reactivity for mouse. The kit is designed for serum, plasma and cell culture supernatants, and the stated assay time is 3 h 30 min. Sensitivity is given as 0.1 ng/mL, the detection range as 0.16-10 ng/mL, and the sample volume as 100 μL. CTSB is also listed under the synonyms APPS and CPSB.

The manufacturer states that the kit recognizes mouse CTSB and that no significant cross-reactivity or interference between mouse CTSB and analogues was observed. Overall recovery is listed as 80%-120%; in the sample-specific study, EDTA plasma showed an average recovery of 91% with a range of 85-99%, and cell culture media showed an average of 95% with a range of 90-104%. Intra-assay CV values were 4.29%, 5.9% and 5.96%, and inter-assay CV values were 6.59%, 8.87% and 8.2%. Dilution linearity was reported for serum, EDTA plasma and cell culture media at 1:2, 1:4, 1:8 and 1:16.

The kit includes a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, biotinylated detection antibody diluent, HRP conjugate diluent, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Storage is 2-8°C. The listed research areas are cancer, neuroscience and signal transduction.