Mouse CTLA4 (Cytotoxic T-Lymphocyte Associated Antigen 4) ELISA Kit (MOES00949)

Quantitative measurement of mouse cytotoxic T-lymphocyte associated antigen 4 (CTLA-4) is enabled by this Assay Genie microplate immunoassay configured for standard laboratory absorbance reading. The matched antibody pair workflow captures CTLA-4 from rodent research specimens, including serum samples, EDTA plasma aliquots, and tissue culture supernatant harvests taken from in vitro cell culture work or from in vivo murine model studies, and produces a colorimetric signal proportional to analyte concentration. Each kit supplies sufficient reagents to perform 96 assays in plate format, supporting mid-throughput screening of experimental cohorts.

The assay supports immunology and cancer immunotherapy research focused on immune checkpoint regulation, including characterization of regulatory T-cell signaling, autoimmune disease progression studies, transplant tolerance investigation, and pharmacodynamic profiling of experimental biologics. The reagent bundle includes pre-coated plate wells, biotinylated detector antibody, recombinant CTLA-4 standards, streptavidin conjugate, substrate solution, and washing buffer required for a full quantitative standard curve. Reconstituted components require refrigerated storage at 4 degrees C and should be protected from light to maintain antibody binding activity; the product is intended for research use only and is classified for in vitro laboratory applications. To obtain reliable results, unknowns are run on the same plate as a freshly prepared standard curve and interpolated against the resulting calibration line, with sample volume held consistent across wells to limit edge effects.