Mouse Creatine Kinase, Brain (CKB) ELISA Kit

The Mouse Creatine Kinase, Brain (CKB) ELISA Kit from Assay Genie quantitatively measures CKB, a cytoplasmic isoform of creatine kinase that catalyzes the reversible transfer of a phosphate group between ATP and creatine. CKB supports cellular energy buffering through the phosphocreatine shuttle and is expressed at high levels in brain, smooth muscle, and many peripheral tissues, making the kit useful for neuroscience, cardiac, skeletal muscle, and oncology research workflows.

The assay accommodates tissue homogenates, cell lysates, and clarified specimen preparations. Each kit includes a pre-coated microplate, biotin-conjugated detection antibody, streptavidin-HRP reagents, calibrated standards, wash buffer, and substrate solution for one plate run. Researchers typically load 50 µL of standard or sample per well and complete the full protocol in roughly three hours at room temperature. Unopened kits should be stored refrigerated; reconstituted reagents are stable short term at -20 °C and tolerate up to three freeze-thaw cycles.

Typical applications include profiling CKB expression in mouse models of neurological disease, ischemic injury, muscular dystrophy, and tumor metabolism. The kit is supplied for research use only and is not intended for diagnostic procedures.