Mouse Cortisol PharmaGenie ELISA Kit (SBRS0047)

Competitive ELISA reagents targeting mouse cortisol support research into hypothalamic-pituitary-adrenal axis regulation, adrenal function, glucocorticoid-mediated stress responses, and metabolic disease modeling. The format relies on antibody competition between sample cortisol and a labeled cortisol conjugate, with colorimetric readout on standard microplate readers. A complete reagent set covering 96 assays is supplied by Assay Genie.

Sample matrix flexibility spans serum-derived preparations, plasma aliquots, and cell culture supernatant fractions, accommodating both whole-animal sampling workflows and in vitro glucocorticoid release experiments. Kit components require refrigerated storage between uses; substrate and conjugate working solutions should be handled protected from light. Throughput integrates cleanly with common automated plate washers. For research use only.

Mouse cortisol measurement is commonly paired with ACTH and corticosterone time-course profiling, behavioral stress endpoints, and pharmacologic intervention studies. The competitive immunoassay format is the established method for small steroid quantification, since two non-overlapping antibody epitopes are typically not available on molecules of this size.