Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit

The Mouse Corticotropin Releasing Hormone (CRH) competitive ELISA from Assay Genie provides quantitative detection of CRH peptide across mouse-derived sample types. Built around a microplate pre-coated with capture antibody specific to mouse CRH, the assay uses an HRP-conjugated reporter and a colorimetric substrate to deliver an inverse signal proportional to analyte concentration. The competitive format is preferred for CRH because the small peptide cannot be reliably captured between two antibodies in a sandwich configuration.

The standard curve spans a detection range 15.6 to 1000 pg/mL with sensitivity 1.56 pg/mL, supporting measurement of basal and stimulated hormone levels in stress-response studies. Each well requires a 50 µL sample volume, and the full protocol completes in approximately 4.5 hours from plate setup to final read. The kit accommodates serum, plasma, and tissue culture supernatant workflows used in mouse endocrinology and HPA axis research.

Components are supplied in a 96-well format kit containing pre-coated plate, lyophilized CRH standards, detection antibody, wash buffer, TMB substrate, and stop solution. Reagents should be refrigerated to preserve activity through the stated shelf life, and the product is intended for research use only.