Mouse Coronin-1A (Coro1a) ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the precise measurement of Coronin-1A levels in mouse serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Using a colorimetric detection method, the assay provides high specificity for natural and recombinant mouse Coronin-1A, also known by synonyms including Coronin-like protein A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein, Clipin-A, TACO, and Coro1 (UniProt O89053).

Performance specifications include a detection range of 0.156-10ng/mL and a sensitivity of 0.076ng/mL. Precision is characterized by an intra-assay CV of 8.3% and inter-assay CV of 10.3%. Linearity has been validated across serum, EDTA plasma, and heparin plasma samples at dilutions of 1:2, 1:4, 1:8, and 1:16, with the reported percentage ranges varying by sample matrix. Recovery is reported at an average of 105% (range 99-111%) for serum and 107% (range 101-113%) for plasma.

Coronin-1A is a member of the WD repeat coronin family and a critical protein involved in cellular processes such as actin cytoskeleton rearrangement and immune response regulation, playing a significant role in autoimmune diseases and cancer research. Functionally, it is considered a crucial cytoskeletal component of highly motile cells involved in plasma membrane invagination and cell locomotion. The kit is designated for research use only within the immunology research area.