Mouse Connexin 40/GJA5 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit uses a double-antibody format with colorimetric detection to quantify Mouse Connexin 40 (GJA5) in research samples. Connexin-40 is a gap junction protein involved in intercellular communication within the cardiovascular system, contributing to coordination of heart rhythm and blood flow, and its dysfunction has been linked to various cardiovascular diseases. The target is associated with Uniprot accession Q01231 and synonyms including CX40, ATFB11, Cx40, and gap junction alpha-5 protein.

Validated for mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, and other liquid samples, the assay delivers a sensitivity of 11.719 pg/mL and a measuring range of 19.531-1250 pg/mL. Reagents are stored at 2-8°C for 12 months, and absorbance is read at 450 nm after TMB color development followed by stop solution.

Reported linearity values for the kit span 85-105% (Serum), 85-100% (EDTA Plasma), and 80-97% (Heparin Plasma) across 1:2, 1:4, and 1:8 sample dilutions. Spike-and-recovery performance is 88-104% (average 98%) for Serum, 85-102% (average 94%) for EDTA Plasma, and 91-105% (average 100%) for Heparin Plasma.