Mouse Connexin 26/GJB2 ELISA Kit

From Assay Genie, the Mouse Connexin 26 (GJB2) ELISA Kit is a Sandwich ELISA employing a double-antibody format and a colorimetric detection method for the precise quantification of Connexin 26 levels in mouse samples. The kit has a stated sensitivity of 11.719 pg/mL and a working range of 19.531-1250 pg/mL, and is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. Components should be stored at 2-8°C for 12 months.

Connexin 26 is described as a vital protein involved in cell communication, playing a critical role in the development and function of various tissues including the inner ear and skin. Mutations in the GJB2 gene encoding Connexin 26 have been linked to sensorineural hearing loss and skin disorders, making the protein a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. The associated UniProt entry is Q00977, and the kit falls within the Cell Biology research area, with CX26 listed as a synonym.

Validation data for the assay include linearity results across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, as well as recovery figures for the same sample types. The procedure is a sequential incubation protocol with reagent equilibration, primary antigen binding at 37°C for 90 minutes, biotin-labelled detection antibody incubation, HRP-streptavidin binding, TMB colour development, and absorbance reading at 450 nm.