Mouse Complement Component 5a (C5a) ELISA Kit

Quantify mouse complement component 5a with this ELISA kit from Assay Genie. C5a is a 74 amino acid anaphylatoxin generated when complement component C5 is cleaved by C5 convertase during activation of the complement cascade. It acts as a potent pro-inflammatory mediator through binding to the C5a receptor (C5aR/CD88) on immune cells including neutrophils, macrophages, and mast cells.

The kit is configured in a microplate format and uses an absorbance-based readout for measuring native and recombinant C5a in mouse samples. Researchers studying inflammation, sepsis, autoimmune disease, transplant rejection, and complement-mediated injury apply the assay to a panel of murine sample types including serum-type specimens, plasma-type specimens, tissue homogenate preparations, cell lysate preparations, and cell culture supernatant specimens obtained from mouse studies.

Reactivity is specific to mouse, supporting translational research that uses murine models of human complement-related disease. The kit includes pre-coated wells, detection antibodies, buffers, and recombinant standard for generating a calibration curve. Reagents should be stored refrigerated to preserve binding activity and assay performance.