Mouse Complement Component 4b (C4b) ELISA Kit

A quantitative immunoassay from Assay Genie, the Mouse Complement Component 4b (C4b) ELISA Kit enables researchers to measure the C4b activation fragment of complement in mouse specimens. C4b is produced during classical and lectin pathway activation when native C4 is cleaved, and tracking its concentration provides insight into complement-driven inflammation, immune complex disease, and autoimmune models including lupus and rheumatoid arthritis.

This microplate-format research use only kit is suited for laboratory studies of the murine complement system. Each kit supplies reagents for a complete microplate run, and the workflow fits standard plate readers commonly available in immunology labs. Reagents should be refrigerated upon arrival; users should avoid repeated freeze-thaw cycles of working dilutions to preserve assay performance.

The assay is often paired with western blot validation of complement proteins in tissue lysates, or with immunohistochemistry to localise C4b deposition in kidney, skin, or synovial sections from experimental mouse models. It is a useful tool for vaccine research, transplant studies, and investigations of infectious disease models where complement consumption is a feature.