Mouse Complement C3/C3 ELISA Kit

This Assay Genie microplate immunoassay quantitatively measures complement component 3 (C3), the central hub protein of the mouse complement cascade. As the convergence point of the classical, lectin, and alternative activation pathways, C3 circulates at high abundance and serves as a primary read-out of systemic complement activity in murine studies.

Run as a colorimetric two-antibody binding assay on a standard microplate strip format, the kit supplies reagents for 96 individual determinations. Small volumes of mouse blood-derived specimens are loaded directly into pre-coated wells, with calibrators and internal controls supplied to generate an absolute standard curve for quantification.

Typical applications include research into autoimmune disease models such as lupus and membranous nephritis, sepsis and acute inflammation studies, transplant rejection, ischemic-reperfusion injury, and characterization of complement-deficient mouse strains. Investigators use the assay to track C3 turnover, monitor therapeutic interventions, and stratify experimental cohorts by baseline complement activity in wild-type versus knockout animals. All reagents should be stored refrigerated and protected from light to preserve activity through the shelf life of the kit.