Mouse COMP/Cartilage oligomeric matrix protein ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA is designed for the precise measurement of Cartilage Oligomeric Matrix Protein (COMP) levels in mouse samples. COMP is an important protein found in cartilage tissue that contributes to its structure and function. Changes in COMP levels have been linked to conditions such as osteoarthritis, rheumatoid arthritis, and other joint disorders, making it a valuable biomarker for studying these diseases and exploring potential treatment options.

The kit is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It offers a sensitivity of 0.938 ng/mL and a detection range of 1.563-100 ng/mL, using a double-antibody sandwich detection method. Reagents should be stored at 2-8°C for 12 months. The associated UniProt ID is Q9R0G6.

Validated recovery rates are 87-104% (average 92%) in serum, 85-105% (average 92%) in EDTA plasma, and 87-102% (average 96%) in heparin plasma. Linearity across dilutions of 1:2, 1:4, and 1:8 falls between 85% and 101% across these sample types. The six-step assay procedure includes a 90-minute primary incubation at 37°C, a 60-minute detection antibody binding step, a 30-minute HRP-streptavidin (SABC) incubation, TMB substrate color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm.