Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

The Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit from Assay Genie is a quantitative sandwich immunoassay for measuring G-CSF (CSF3) levels in mouse samples. G-CSF is a 19 kDa glycoprotein cytokine that drives neutrophil production, differentiation, and mobilization from the bone marrow, making it a key biomarker in studies of hematopoiesis, infection, inflammation, and chemotherapy-induced neutropenia.

This 96-well microplate kit delivers a colorimetric readout and is validated for serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. Typical performance includes a standard detection range of 15.6 to 1000 pg/mL and a sensitivity below 10 pg/mL, using a sample volume of approximately 50 uL per well.

Reagents are shipped refrigerated and should be stored at 2 to 8 degrees C until first use; reconstituted standards and detection antibody are kept frozen at -20 degrees C to preserve activity across freeze-thaw cycles. The kit is supplied with a pre-coated microplate, biotinylated detection antibody, HRP-streptavidin conjugate, calibrated standards, and assay diluents, supporting research use only workflows such as cytokine profiling in murine models of sepsis, bone marrow recovery, and granulopoiesis.