Mouse Collagen alpha-1 (XVII)chain (Col17a1) ELISA Kit

Assay Genie offers an immunoassay kit for the quantitative determination of mouse Collagen alpha-1(XVII) chain (COL17A1). Source documents describe the assay as both a sandwich ELISA with colorimetric detection and a competitive ELISA with ELISA detection. It is reactive with mouse samples and has been validated for serum and plasma; manufacturer information additionally lists tissue homogenates, cell culture supernates and other biological fluids. Specificity is reported for natural and recombinant mouse COL17A1, also referred to as BP180 or 180 kDa bullous pemphigoid antigen 2.

Manufacturer figures quote a detection range of 0.312–20 ng/mL, a sensitivity of 0.156 ng/mL, an intra-assay CV of 4.9% and an inter-assay CV of 7.4%. Linearity in serum across dilutions 1:2, 1:4, 1:8 and 1:16 is given as 99–109%, 91–100%, 94–104% and 82–92% respectively; EDTA plasma values are 98–110%, 99–109%, 80–90% and 101–111%; heparin plasma values are 98–109%, 91–99%, 94–103% and 103–115%. Recovery is listed as 95% (range 89–101%) for serum and 97% (range 91–103%) for plasma. The associated UniProt accession is Q07563.

COL17A1 is described as a component of the hemidesmosomal anchoring filament complex involved in dermal-epidermal cohesion. Reported molecular weight is 144,140 Da, with the NCBI gene symbol Col17a1 (synonyms Bpag, BP180, Bpag2) and UniProt entry name COHA1_MOUSE. The product is labelled for research use only.