Mouse Collagen alpha-1 (X)chain (Col10a1) ELISA Kit

Col10a1 (Collagen type X alpha 1 chain) is a short-chain collagen expressed almost exclusively by hypertrophic chondrocytes, and is widely used as a molecular marker of chondrocyte hypertrophy, terminal differentiation, and endochondral ossification. This quantitative immunoassay from Assay Genie enables researchers to measure murine Col10a1 protein levels in experimental samples, supporting studies of skeletal development, growth plate maturation, osteoarthritis progression, and cartilage-to-bone transition during fracture repair.

The complete 96-assay microplate-based kit includes pre-coated wells, lyophilized standards, a labelled detection antibody, substrate, and wash buffer sufficient to run unknown samples alongside a standard curve. Each plate is processed under standard in vitro ELISA conditions and the assay is calibrated for use with mouse-derived tissue homogenates, cell lysates, and blood-derived specimens collected during in vivo studies.

Intended for research use only, the kit should be stored refrigerated at 4 degrees C to maintain antibody and standard stability through the stated shelf life. The protocol accommodates batch comparison of Col10a1 expression across transgenic strains, developmental time courses, and treatment cohorts investigating cartilage biology and bone formation.