Mouse Collagen alpha-1 (II)chain (Col2a1) ELISA Kit

From Assay Genie, this Sandwich ELISA is configured for mouse reactivity and targets Collagen alpha-1(II) chain (Col2a1), also referred to by the synonym Alpha-1 type II collagen. The kit is for research use only and is intended to detect natural and recombinant mouse Collagen alpha-1(II) chain in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

The detection method is ELISA, with a stated detection range of 0.156-10ng/mL and a sensitivity of 0.089ng/mL. According to the manufacturer, intra-assay CV, inter-assay CV, linearity, and recovery data are provided with the kit. The associated UniProt entry is P28481. The target protein functions as homotrimers of alpha 1(II) chains, is secreted, and localises to the extracellular space and extracellular matrix. Type II collagen is described by the manufacturer as specific for cartilaginous tissues and essential for the normal embryonic development of the skeleton, for linear growth, and for the ability of cartilage to resist compressive forces.

Kit components include a dismountable ELISA microplate, lyophilised standard, sample diluent, assay diluent A, assay diluent B, detection reagent A, detection reagent B, wash buffer, substrate solution, stop solution, and plate sealers, with component storage temperatures ranging between -20°C and 4°C. The Research Area is listed as Signal Transduction.