Mouse COL1 alpha2 (Collagen Type I Alpha 2) ELISA Kit (MOES00882)

The Assay Genie sandwich ELISA measures native and recombinant mouse Collagen Type I Alpha 2 (COL1A2) in serum, plasma, and other biological fluids using a 96-well microplate pre-coated with capture antibody. Standards and samples are added to wells, followed by a biotinylated detection antibody and streptavidin-HRP conjugate; color develops with TMB substrate and is read at 450 nm. Typical assay range covers 0.156-10 ng/mL with sensitivity around 0.05 ng/mL. The kit includes pre-coated plate, standards, detection antibody, HRP conjugate, substrate, wash buffer, and stop solution. Store all components at 2-8°C, protect from light, and avoid repeated freeze-thaw cycles. Researchers commonly apply this kit in fibrosis, wound healing, bone formation, and extracellular matrix studies.