Mouse Cofilin-2 (Cfl2) ELISA Kit

Assay Genie provides a Sandwich ELISA kit for the quantitative detection of Cofilin-2 (Cfl2) in mouse samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay is reactive with mouse samples and recognises natural and recombinant mouse Cofilin-2, offering a detection range of 0.312–20 ng/mL and a sensitivity of 0.156 ng/mL.

According to Assay Genie, Cofilin-2 is a key protein involved in actin dynamics, playing a critical role in cell migration, cytoskeletal organization, and cell membrane protrusions. The manufacturer states that dysregulation of cofilin-2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. The kit is for research use only and is associated with the Signal Transduction research area. The target protein is catalogued at UniProt P45591, with isoform b expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

The workflow follows a standard sandwich ELISA format in which absorbance is read at 450 nm. The protein is described as a major component of intranuclear and cytoplasmic actin rods, required for muscle maintenance, with the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin and to control reversibly actin polymerization and depolymerization in a pH-sensitive manner.