Mouse Coagulation Factor II (F2) ELISA Kit

Prothrombin, the vitamin K-dependent zymogen precursor of thrombin, occupies a central position in the coagulation cascade and is a key quantitative readout in murine models of hemostasis, thrombotic disease, and anticoagulant pharmacology. The Assay Genie Mouse Coagulation Factor II (F2) ELISA Kit delivers a 96-assay plate format validated specifically for mouse plasma, with colorimetric measurement on standard microplate readers and pre-coated wells supporting duplicate standard-curve points alongside biological replicates on a single plate.

Each kit is intended for research use only and supports laboratory investigation of coagulation biology, including studies of bleeding disorders, vitamin K-dependent carboxylation, hepatic synthesis of clotting factors, and the impact of anticoagulant or procoagulant interventions in murine disease models. The plasma-validated protocol enables direct quantification of circulating prothrombin without extensive sample preprocessing, and the plate layout allows simultaneous evaluation of multiple treatment groups, time points, or genetic backgrounds.

Reagents ship under refrigeration and should be stored refrigerated upon arrival, with standards and detection antibody brought to room temperature before reconstitution per the supplied protocol. Reconstituted components remain stable under refrigerated storage through the period specified in the lot-specific certificate of analysis. Quality-control testing covers intra-assay and inter-assay precision against reference mouse plasma, providing reproducible quantification across experimental batches.