Mouse CNTN4 ( Contactin 4) ELISA Kit (MOES01805)

The Assay Genie Mouse CNTN4 (Contactin 4) ELISA Kit pairs a pre-coated capture antibody with a biotinylated detection reagent and an HRP-streptavidin reporting step, allowing 100 µL of neat or diluted sample per well. Reconstituted standards should be aliquoted and held frozen at -20°C to preserve the calibration curve, while the sealed microplate, concentrated detection antibody, and wash buffer remain stable under refrigeration for up to 12 months from the date of manufacture. Contactin 4, also known by the synonym BIG-2 in the immunoglobulin superfamily, maps to mouse chromosome 6 band E1 (UniProt Q69Z26) and is recognized in both native and recombinant forms, with no significant cross-reactivity observed against recombinant human CNTN1, CNTN2, or CNTN6 paralogs. Researchers investigating autism spectrum disorder, 3p deletion syndrome, or cerebellar granule cell migration can apply the assay to cell culture supernatant, tissue homogenate, and matched serum or EDTA plasma samples to quantify changes associated with axon guidance, neuronal migration, and synapse formation in the developing central nervous system.