Mouse Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

Quantifying soluble mouse CD40 ligand (CD40L), the costimulatory molecule also designated CD154 and TNFSF5, this Assay Genie Mouse CD40L ELISA Kit provides an in vitro quantitative immunoassay suited to immunology, inflammation, transplant, and vaccine research workflows. The ligand, expressed predominantly on activated CD4+ T cells, engages CD40 on B lymphocytes, dendritic cells, and macrophages to drive germinal center formation, immunoglobulin class switching, and cytokine production — processes relevant to autoimmune models, infectious disease studies, and immunogenicity assessments.

Each kit contains pre-coated microplate wells, detection reagent, standards, substrate, and buffers packaged in individual vials, supporting 96 individual determinations per run. The assay is validated for mouse reactivity and is compatible with multiple mouse sample matrices including tissue homogenates and cell lysates, broadening its application across experimental specimen types. Refrigerated storage preserves reagent stability across the working session, and the standard microplate-based protocol integrates readily with existing plate-reader absorbance workflows for routine bench use.