Mouse Chromosome 1 Open Reading Frame 85 (C1orf85) ELISA Kit

The Mouse Chromosome 1 Open Reading Frame 85 (C1orf85) ELISA Kit from Assay Genie is supplied as a complete microplate-based quantitative immunoassay reagent set for measuring endogenous C1orf85 concentrations in prepared mouse samples. C1orf85 is a predicted protein-coding locus whose expression pattern is increasingly studied in murine models of tissue development, with researchers using quantitative immunoassays to track translational output in lysates from heart, liver, kidney and brain.

The kit contains a pre-coated microplate, lyophilized reference standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, substrate solution, stop solution and assay diluent. Researchers typically load 100 µL of standard or diluted sample per well, with an incubation schedule of approximately 2 hours at room temperature before signal development. All reagents should be stored at -20 °C upon arrival to preserve antibody activity, and the kit is intended for research use only in laboratory settings, not for diagnostic procedures.

Assay Genie provides lot-specific validation data and a detailed protocol insert. The microplate format supports standard curve generation in duplicate, allowing an eight-point dilution series alongside unknown samples, which is useful for small cohort studies and exploratory expression profiling where lysate volume is constrained.