Mouse Choline Acetyltransferase (ChAT) ELISA Kit

Engineered by Assay Genie for neuroscience research laboratories, this Mouse Choline Acetyltransferase (ChAT) ELISA Kit delivers a quantitative, plate-based immunoassay to measure ChAT, the acetylcholine-synthesizing enzyme that converts acetyl-CoA and choline into acetylcholine. The kit is calibrated exclusively for mouse samples, supporting investigations of cholinergic neuron function, neuromuscular junction biology, and neurodegeneration models relevant to cognitive decline, Alzheimer's disease, amyotrophic lateral sclerosis, and autonomic nervous system disorders. Each kit supplies reagents sufficient for 96 determinations across a microplate, with an absorbance-based readout compatible with standard laboratory plate readers.

Compatible sample matrices include tissue homogenates prepared from brain regions such as the cortex, hippocampus, and basal forebrain, alongside cell lysates from cultured neurons or cholinergic cell lines. Researchers commonly apply this kit to track ChAT expression in transgenic mouse models, evaluate pharmacological modulation of cholinergic signaling, and compare enzyme levels across developmental stages or disease states. All buffers, standards, detection reagents, and substrate components needed for the complete workflow are included to minimize hands-on preparation. Each batch is validated for intra- and inter-assay precision to support reproducible longitudinal data.

For research use only, this microplate-format kit offers a practical tool for laboratories studying acetylcholine metabolism, synaptic plasticity, and the molecular mechanisms underlying cognition, memory, and neurological disease progression.