Mouse Cholesterol side-chain cleavage enzyme, mitochondrial (Cyp11a1) ELISA Kit

A sandwich-format colorimetric ELISA from Assay Genie, this kit enables quantitative detection of mouse cholesterol side-chain cleavage enzyme, mitochondrial (Cyp11a1). The target protein, also known as CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, and Cytochrome P450(scc), is a mitochondrial membrane enzyme that catalyzes the side-chain cleavage of cholesterol to pregnenolone — a key step in steroid hormone biosynthesis — and is documented to interact with FDX1/adrenodoxin.

Validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, the assay specifically recognises natural and recombinant mouse Cyp11a1. It is intended for research use only and is positioned within cardiovascular research, including studies on adrenal insufficiency and reproductive disorders associated with CYP11A1 dysregulation.

Manufacturer-reported performance figures include a detection range of 0.78–50 ng/mL and a sensitivity of 0.405 ng/mL. The corresponding UniProt entry is Q9QZ82 (NCBI Gene ID: 13070), with the protein reported at a molecular weight of 60,315 Da.