Mouse Cellular retinoic acid-binding protein 2 (Crabp2) ELISA Kit

From Assay Genie, this colorimetric Sandwich ELISA kit is designed for the accurate detection of Mouse Cellular retinoic acid-binding protein 2 (CRABP2) levels in mouse serum, plasma, and cell culture supernatants. CRABP2 is a critical protein involved in retinoic acid signaling, where it transports retinoic acid to the nucleus and regulates access of retinoic acid to the nuclear retinoic acid receptors. The kit is specific for natural and recombinant mouse CRABP2, making it a valuable tool for research into cell growth, differentiation, and development, as well as conditions where CRABP2 dysregulation has been implicated, including cancer, metabolic disorders, and skin diseases.

Quantitative performance characteristics include a detection range of 0.156-10 ng/mL and a sensitivity of 0.087 ng/mL, with an intra-assay CV of 4.9% and an inter-assay CV of 8.2%. Linearity was validated in serum (N=5) at 106-116%, 102-111%, 96-107%, and 92-102% across 1:2, 1:4, 1:8, and 1:16 dilutions; in EDTA plasma (N=5) at 88-96%, 108-118%, 103-114%, and 93-103%; and in heparin plasma (N=5) at 114-124%, 106-116%, 97-107%, and 89-98%. Recovery averages 103% (range 97-109%) in serum and 105% (range 99-111%) in plasma. The target protein (UniProt P22935; NCBI Gene ID 12904) has a molecular weight of 15,746 Da, belongs to the calycin superfamily, fatty-acid binding protein (FABP) family, and is located in the cytoplasm, endoplasmic reticulum, and nucleus.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The kit is for research use only.