Mouse CD62P/P-selectin ELISA Kit

Designed for immunology, cardiovascular, and inflammation research, this Sandwich ELISA Kit from Assay Genie enables the sensitive and accurate quantification of mouse CD62P (P-selectin), a cell adhesion molecule involved in leukocyte recruitment and thrombus formation. The double-antibody sandwich assay is applicable to serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed for mouse.

Key performance characteristics include a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. Reported linearity at a 1:2 dilution spans 87-103% for serum, 88-104% for EDTA plasma, and 85-96% for heparin plasma, with recovery ranges of 86-104% (average 97%) for serum, 86-104% (average 95%) for EDTA plasma, and 85-104% (average 92%) for heparin plasma. Associated UniProt reference: Q01102.

The protocol uses a pre-coated plate, biotin-labelled detection antibody, HRP-streptavidin conjugate (SABC), and TMB substrate, with absorbance measured at 450 nm. Storage is at 2-8°C for 12 months.