Mouse CD6 PharmaGenie ELISA Kit (SBRS1310)

The Mouse CD6 PharmaGenie ELISA Kit from Assay Genie quantitatively measures the CD6 glycoprotein, a 105-130 kDa scavenger receptor cysteine-rich (SRCR) superfamily member expressed on thymocytes, mature peripheral T lymphocytes, and a subset of B1a B cells. CD6 engages the ALCAM (CD166) ligand and contributes to T-cell maturation, adhesive contact at the immunological synapse, and modulation of TCR signaling strength.

This immunoassay from Assay Genie requires only a 15 µL sample volume per well, distinguishing it from conventional plate immunoassays that typically consume 50-100 µL. The procedure uses pre-coated capture plates, an HRP-conjugated detection antibody, and a TMB substrate revealed by absorbance measurement. Researchers can therefore profile CD6 levels across experimental conditions using minimal mouse-derived matrices such as cell culture supernatant, along with compatible handling of plasma and serum specimens.

Typical research applications include tracking T-cell activation kinetics in murine autoimmune disease models such as EAE and collagen-induced arthritis, lymphocyte development studies, and immune checkpoint investigations involving the CD6-ALCAM axis. The kit is intended for research use only; store components refrigerated between runs to preserve antibody binding activity through the stated shelf life, and avoid repeated freeze-thaw cycles of reconstituted standards.