Mouse CD5 antigen-like (Cd5l) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative detection of mouse CD5 antigen-like (Cd5l). The assay is validated for use with mouse samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and uses a colorimetric detection method. Synonyms associated with the target include apoptosis inhibitor expressed by macrophages (AIM), apoptosis inhibitory 6 (Api6), and SP-alpha.

Stated performance figures include a detection range of 31.2-2000 pg/mL and a sensitivity of 15.63 pg/mL. Intra-assay precision is reported as 4.3% CV and inter-assay precision as 8.5% CV. Reported linearity in serum spans 93-103% at 1:2 dilution to 96-108% at 1:16; EDTA plasma ranges from 91-103% to 93-105%, and heparin plasma from 94-105% to 99-111% over the same dilution series. Recovery averages 99% for serum (range 93-105%) and 101% for plasma (range 95-107%). Specificity is described for both natural and recombinant mouse CD5 antigen-like.

According to the supplied information, CD5L is a secreted protein mainly expressed by macrophages in lymphoid and inflamed tissues, acting as a key regulator of lipid synthesis and as an inhibitor of apoptosis in macrophages, with described roles in inflammatory responses including infection and atherosclerosis. The product is supplied for research use only.