Mouse CD47 PharmaGenie ELISA Kit (SBRS1307)

The Mouse CD47 PharmaGenie ELISA Kit from Assay Genie is built on a sandwich immunoassay architecture using a pre-coated microplate, enabling quantitative measurement of CD47 (integrin-associated protein) in mouse-derived samples. CD47 is a transmembrane receptor best known for its role as a "don't eat me" signal through interaction with SIRPα on macrophages, making accurate quantification relevant to studies of tumor immune evasion, phagocytosis regulation, and hematopoietic cell signaling. The kit uses mouse-specific capture and detection antibodies and shows no significant cross-reactivity with related immunoglobulin superfamily members, supporting research in immunology, oncology, and cell biology. The 96-well microplate format allows parallel processing of standards and unknowns, with a colorimetric endpoint readable on standard plate readers. The recommended sample volume is 100 µL per well, and assay components should be refrigerated; reconstituted standards and diluted samples should be aliquoted to minimize freeze-thaw cycles before use. Intended for research use only, this kit complements validation workflows including flow cytometry, immunohistochemistry, and western blot comparisons of CD47 expression across mouse model tissues and treatment conditions.