Mouse CD30 L PharmaGenie ELISA Kit (SBRS1302)

Background on CD30 Ligand

CD30 Ligand (CD30L, also known as CD153 or TNFSF8) is a type II transmembrane glycoprotein of the TNF superfamily. It binds the CD30 receptor (TNFRSF8) on target cells, providing costimulatory signals that influence T-cell activation, Th2 polarization, antibody class switching, and apoptosis. CD30L is expressed on activated T cells, B cells, mast cells, eosinophils, and neutrophils, and dysregulation of the CD30/CD30L axis has been implicated in Hodgkin's lymphoma, anaplastic large cell lymphoma, allergic inflammation, and autoimmune disease research.

Kit Performance

• Standard curve: 7-point serial dilution
• Sample volume: 100 µL per well
• Assay time: approximately 3.5 hours
• Compatible with automated plate washers
• Reagents stable through the kit's printed expiration date

Research Applications

This Assay Genie kit is used in immunology and oncology laboratories to quantify soluble CD30L in mouse model samples from studies of lymphoma biology, transplant rejection, allergic airway inflammation, and T-cell costimulation. The kit supports longitudinal biomarker studies, evaluation of monoclonal antibody therapeutics targeting the CD30 axis, and characterization of CD30/CD30L signaling in Th2-driven immune responses.

Storage and Handling

Store unopened components at 4°C; protect the standard from light and avoid repeated freeze-thaw cycles by aliquoting after reconstitution. The microplate is shipped ready to use and all reagents are stable through the expiration date when stored as directed.