Mouse CD163 (Cluster of Differentiation 163) ELISA Kit (MOES01718)

Quantitative measurement of the CD163 scavenger receptor on macrophages and monocytes is enabled by the Assay Genie Mouse CD163 ELISA Kit, which supports studies of inflammation, hemolysis, and macrophage polarization in murine research models. CD163 serves as the high-affinity receptor for hemoglobin-haptoglobin complexes, and its surface expression shifts dynamically with M1-to-M2 macrophage transitions while its shed soluble form (sCD163) accumulates in body fluids during sepsis, atherosclerosis, and non-alcoholic fatty liver disease. Investigators in immunology, hepatology, vascular biology, and infectious disease apply this readout to characterize anti-inflammatory macrophage activation, monocyte recruitment, and heme-iron handling in vivo.

Each package supports 96 individual determinations and supplies the pre-coated microplate, recombinant mouse CD163 calibrator, biotinylated detection antibody, streptavidin-HRP conjugate, TMB chromogen, stop reagent, wash concentrate, and assay diluent needed to complete the workflow. The protocol accommodates mouse serum or plasma specimens, along with cell culture supernatant and tissue lysate inputs, supporting in vitro and in vivo research programs tracking macrophage activation or soluble CD163 release dynamics.