Mouse CAV1 (Caveolin 1) ELISA kit (MOES00823)

Assay Genie supplies this sandwich ELISA as a complete ready-to-use kit containing a pre-coated 96-well microplate, lyophilized recombinant CAV1 standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate, and stop solution sufficient for 96 determinations run in duplicate. The standard curve typically spans approximately 0.16-10 ng/mL, with intra-assay and inter-assay coefficients of variation generally reported below 10%. Sample inputs of 50-100 µL of serum, plasma, or clarified tissue homogenate are usually sufficient after appropriate dilution in the provided diluent. The capture antibody binds full-length CAV1 protein, and selectivity for the CAV1 isoform over related caveolin family members (CAV2, CAV3) supports specific quantitative readouts. Plate layout allows concurrent loading of standards, positive controls, and unknowns, and the full workflow from sample addition to absorbance measurement at 450 nm can be completed within approximately 3-4 hours.