Mouse Caspase 9 (CASP9) ELISA Kit

Apoptosis research in murine models depends on reliable quantification of initiator caspase activity, and this immunoassay from Assay Genie delivers a quantitative measure of mouse CASP9 protein in experimental samples. The kit is configured as a microplate with capacity for 96 assays, supplied with pre-coated wells, capture antibody, detection antibody, standards, and all buffers required to run the full plate. It accepts tissue homogenates and cell lysates prepared from mouse specimens, with the workflow following an incubation-and-wash capture format that uses an enzyme-conjugated reporter and a substrate yielding an absorbance readout measurable on a standard plate reader.

Each plate includes a recombinant mouse caspase 9 standard curve for interpolation of unknown sample concentrations, supporting studies of intrinsic pathway activation, mitochondrial apoptosis, and downstream effector caspase signaling. The format lends itself to medium-throughput experimental designs, such as comparing wild-type and knockout mice, time-course studies of apoptosis induction, or screening pharmacological agents that modulate programmed cell death. The kit is intended for research use only and should be stored refrigerated between uses to preserve reagent activity. Researchers can pair CASP9 measurement with executioner caspase 3/7 assays, cytochrome c release, or viability endpoints to build a more complete picture of apoptotic signaling.