Mouse Caspase 8 (CASP8) ELISA Kit

This colorimetric immunoassay from Assay Genie quantifies mouse caspase-8, a cysteine protease that initiates the extrinsic apoptosis cascade downstream of death receptor activation. Tracking CASP8 protein levels in murine samples supports work on programmed cell death, immune cell homeostasis, and inflammatory responses, complementing functional readouts such as enzymatic activity assays and western blot confirmation of cleavage products.

Validated sample inputs include tissue homogenates, cell lysates, and cell culture supernatants, covering both lysate-based and secreted-fraction experimental workflows. Each kit provides reagents for 96 determinations in a microplate configuration, packaged as a complete set with the precoated plate, calibrated standards, biotinylated detection antibody, and streptavidin-HRP conjugate. The protocol uses standard indirect immunoassay steps with an overnight incubation and color development for absorbance readout on a compatible plate reader.

The kit is intended for research use only in laboratory settings and is stored under refrigerated conditions as detailed in the package insert, with shelf life sufficient to support standard short-term studies. Applications include apoptosis research in oncology models, neurodegeneration studies, and immunology investigations where death receptor signaling is a focus.