Mouse Casein kinase II subunit alpha (Csnk2a1) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative detection of mouse Casein kinase II subunit alpha (CSNK2A1) in biological samples. The assay uses a colorimetric detection method and is compatible with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. It specifically recognizes natural and recombinant mouse Casein kinase II subunit alpha.

Performance characteristics include a detection range of 0.625–40 ng/mL and a sensitivity of 0.312 ng/mL. Intra-assay precision is reported at 7.8% CV and inter-assay precision at 10.3% CV. Linearity has been validated in serum (105–117%, 99–111%, 82–91%, 91–101% across 1:2–1:16 dilutions), EDTA plasma (98–106%, 92–103%, 101–111%, 107–116%), and heparin plasma (97–106%, 95–105%, 81–89%, 82–92%). Recovery averages 81% in serum (range 80–87%) and 83% in plasma (range 80–89%).

CSNK2A1 serves as the catalytic subunit of a constitutively active serine/threonine-protein kinase complex that regulates cell cycle progression, apoptosis, transcription, and circadian function, with documented relevance to cancer research. The protein is located in the nucleus (UniProt Q60737). Recommended storage is -20°C with shipping at +4°C, and the kit is intended for research use only.