Mouse Carboxypeptidase N1 (CPN1) ELISA Kit

This quantitative immunoassay from Assay Genie enables researchers to measure circulating levels of mouse Carboxypeptidase N1, a zinc-dependent plasma metalloprotease that cleaves C-terminal arginine and lysine residues from complement anaphylatoxins (C3a, C5a) and kinins such as bradykinin. Quantifying CPN1 by ELISA supports studies of complement activation, kinin metabolism, acute inflammation, and vascular homeostasis in murine models of disease.

The Mouse Carboxypeptidase N1 (CPN1) ELISA Kit ships in a 96-assay format configured for in vitro research use only. It produces a colorimetric readout on a standard microplate and includes pre-coated wells, lyophilized standards, detection antibody, wash buffer, substrate, and a detailed protocol. The assay is validated for use with serum-derived samples, plasma-derived samples, tissue homogenate preparations, and additional mouse-derived matrices. Working reagents should be kept refrigerated, with standards and concentrated antibody stored frozen for long-term activity.

Common research applications include profiling CPN1 expression in knockout or transgenic mice, monitoring carboxypeptidase pathway activity in models of sepsis, arthritis, hereditary angioedema, or kidney injury, and screening compounds that modulate plasma carboxypeptidase function. Each kit is supplied with reagents sufficient for one full microplate run including a standard curve.