Mouse Carboxylesterase 5A/CES5A ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the precise measurement of Carboxylesterase 5A (CES5A) levels in mouse samples. CES5A is an enzyme involved in the metabolism of various compounds, including drugs and toxins, making it a valuable marker for drug metabolism and toxicity studies. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reagents stored at 2–8°C and not to be cryopreserved.

Performance specifications include a sensitivity of 0.188 ng/mL and a standard curve range of 0.313–20 ng/mL, with absorbance read at 450 nm after a TMB-based colorimetric reaction. Linearity at 1:2, 1:4 and 1:8 dilutions spans 85–101%, 86–100% and 86–93% for serum (n=10); 87–98%, 85–100% and 85–97% for EDTA plasma (n=10); and 94–105%, 86–95% and 83–99% for heparin plasma (n=10). Recovery averages 98% for serum, 91% for EDTA plasma and 95% for heparin plasma, with recovery ranges of 91–105%, 85–105% and 86–105% respectively. Synonyms associated with the target include Ces5a, CES7, Cauxin, and carboxylesterase-like urinary excreted protein homolog (UniProt Q6AW46).