Mouse Carbonic Anhydrase I (CA1) ELISA Kit

Researchers measuring mouse Carbonic Anhydrase I (CA1) protein rely on this immunoassay from Assay Genie to generate reproducible quantitative data. CA1 is a cytosolic zinc metalloenzyme expressed at high levels in erythrocytes and gastrointestinal mucosal epithelium, where it catalyzes the reversible hydration of carbon dioxide to bicarbonate. Studies using this kit address questions of systemic acid-base balance, CO2 and bicarbonate transport, red blood cell turnover, gastrointestinal physiology, ocular lens transparency, and bone remodeling.

The 96-assay format supports mid-throughput experimental designs in academic and pharmaceutical research, including work on carbonic anhydrase involvement in tumor microenvironment biology. Reagents supplied with the kit include pre-coated wells, assay buffer, lyophilized standards, detection antibody, and substrate solution calibrated to produce a standard curve for quantifying CA1 in tissue homogenates, cell lysates, cell culture supernatants, and whole-blood fractions from mouse models. Unopened components should be stored refrigerated to preserve activity throughout the kit's shelf life, and reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles.