Mouse Carbonic anhydrase 9 (Ca9) ELISA Kit

From Assay Genie, this Mouse Carbonic anhydrase 9 (Ca9) ELISA Kit is a Sandwich ELISA designed for the quantitative measurement of CA9 in mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Detection is colorimetric, and the kit is intended for research use only.

CA9 is a single-pass type I membrane protein that catalyses the reversible hydration of carbon dioxide and participates in pH regulation, with described roles in cancer progression and the tumour microenvironment. The assay recognises natural and recombinant mouse Carbonic anhydrase 9, and the target is listed under synonyms including Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN homolog, CA-IX and Car9.

Reported performance characteristics include a detection range of 12.5-800 pg/mL, a sensitivity of 6.25 pg/mL, an intra-assay CV of 6.4% and an inter-assay CV of 8.3%. Linearity in mouse serum across 1:2 to 1:16 dilutions spans 86-95% to 96-106%; EDTA plasma spans 90-100% to 86-86%; heparin plasma spans 94-104% to 97-109%. Recovery averages 99% (range 93-105%) for serum and 101% (range 95-107%) for plasma.